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IHC-P analysis of human colon carcinoma tissue using GTX87369 GCHFR antibody. The picture on the right is blocked with the synthesized peptide.
IHC-P analysis of human colon carcinoma tissue using GTX87369 GCHFR antibody. The picture on the right is blocked with the synthesized peptide.
IHC-P analysis of human colon carcinoma tissue using GTX87369 GCHFR antibody. The picture on the right is blocked with the synthesized peptide.

GCHFR antibody

GTX87369
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetGCHFR
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Overview

  • Supplier
    GeneTex
  • Product Name
    GCHFR antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:50~1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2644
  • Target name
    GCHFR
  • Target description
    GTP cyclohydrolase I feedback regulator
  • Target synonyms
    GFRP; GTP cyclohydrolase 1 feedback regulatory protein; GTP cyclohydrolase I feedback regulatory protein; HsT16933; P35
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30047
  • Protein Name
    GTP cyclohydrolase 1 feedback regulatory protein
  • Scientific Description
    GTP cyclohydrolase I feedback regulatory protein binds to and mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase I. The regulatory protein, GCHFR, consists of a homodimer. It is postulated that GCHFR may play a role in regulating phenylalanine metabolism in the liver and in the production of biogenic amine neurotransmitters and nitric oxide. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GCHFR using anti-GCHFR antibody (A08185-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: human HELA whole cell lysates, Lane 6: human U937 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: rat liver tissue lysates, Lane 9: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCHFR antigen affinity purified polyclonal antibody (Catalog # A08185-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCHFR at approximately 12KD. The expected band size for GCHFR is at 12KD.
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