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Western Blot analysis of NIH-3T3 cells using GCK Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using GCK Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using GCK Polyclonal Antibody

GCK Antibody

CSB-PA002656
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGCK
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Overview

  • Supplier
    Cusabio
  • Product Name
    GCK Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2645
  • Target name
    GCK
  • Target description
    glucokinase
  • Target synonyms
    ATP:D-hexose 6-phosphotransferase; FGQTL3; GK; GLK; glucokinase (hexokinase 4); hexokinase D, pancreatic isozyme; hexokinase type IV; hexokinase-4; HHF3; HK IV; HK4; HKIV; HXKP; LGLK; MODY2; PNDM1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35557
  • Protein Name
    Glucokinase
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GCK/Glucokinase Polyclonal Antibody(bs-1796R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Figure 1. Western blot analysis of Glucokinase using anti-Glucokinase antibody (A00884-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glucokinase antigen affinity purified polyclonal antibody (Catalog # A00884-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glucokinase at approximately 52KD. The expected band size for Glucokinase is at 52KD.
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