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WB analysis of rat liver lysate using GTX88432 GCKR antibody, N-term. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of rat liver lysate using GTX88432 GCKR antibody, N-term. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of rat liver lysate using GTX88432 GCKR antibody, N-term. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer

GCKR antibody, N-term

GTX88432
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetGCKR
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Overview

  • Supplier
    GeneTex
  • Product Name
    GCKR antibody, N-term
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 0.1-0.3microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2646
  • Target name
    GCKR
  • Target description
    glucokinase regulator
  • Target synonyms
    FGQTL5, GKRP, glucokinase regulatory protein, glucokinase (hexokinase 4) regulator, hexokinase 4 regulator
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ14397
  • Protein Name
    Glucokinase regulatory protein
  • Scientific Description
    This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY). [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of GCKR using anti-GCKR antibody (A02122-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCKR antigen affinity purified polyclonal antibody (Catalog # A02122-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCKR at approximately 69 kDa. The expected band size for GCKR is at 69 kDa.
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Lane 1: Mouse Liver lysates; Lane 2: U87MG cell lysates; Lane 3: HepG2 cell lysates; Lane 4: A549 cell lysates probed with GKRP Polyclonal Antibody, Unconjugated (bs-2356R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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