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ProductsBack/GCKR Mouse Monoclonal Antibody [Clone ID: OTI9G7]
Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human GCKR plasmid (RC214230, hGCKR), mouse GCKR plasmid (MR209160, mGCKR) using anti-GCKR antibody TA502163 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)
Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human GCKR plasmid (RC214230, hGCKR), mouse GCKR plasmid (MR209160, mGCKR) using anti-GCKR antibody TA502163 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)
Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human GCKR plasmid (RC214230, hGCKR), mouse GCKR plasmid (MR209160, mGCKR) using anti-GCKR antibody TA502163 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

GCKR Mouse Monoclonal Antibody [Clone ID: OTI9G7]

CF502163
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGCKR
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    GCKR Mouse Monoclonal Antibody [Clone ID: OTI9G7]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI9G7
  • Gene ID2646
  • Target name
    GCKR
  • Target description
    glucokinase regulator
  • Target synonyms
    FGQTL5, GKRP, glucokinase regulatory protein, glucokinase (hexokinase 4) regulator, hexokinase 4 regulator
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ14397
  • Protein Name
    Glucokinase regulatory protein
  • Scientific Description
    Carrier-free (BSA/glycerol-free) GCKR mouse monoclonal antibody, clone OTI9G7 (formerly 9G7)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GCKR using anti-GCKR antibody (A02122-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCKR antigen affinity purified polyclonal antibody (Catalog # A02122-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCKR at approximately 69 kDa. The expected band size for GCKR is at 69 kDa.
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