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Western Blot analysis of A549 cells using GCSc-gamma Polyclonal Antibody
Western Blot analysis of A549 cells using GCSc-gamma Polyclonal Antibody
Western Blot analysis of A549 cells using GCSc-gamma Polyclonal Antibody

GCLC Antibody

CSB-PA002665
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGCLC
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Overview

  • Supplier
    Cusabio
  • Product Name
    GCLC Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2729
  • Target name
    GCLC
  • Target description
    glutamate-cysteine ligase catalytic subunit
  • Target synonyms
    CNSHA7, GCL, GCS, GLCL, GLCLC, glutamate--cysteine ligase catalytic subunit, GCS heavy chain, gamma-ECS, gamma-glutamylcysteine synthetase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48506
  • Protein Name
    Glutamate--cysteine ligase catalytic subunit
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GCLC using anti-GCLC antibody (A01722). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCLC antigen affinity purified polyclonal antibody (Catalog # A01722) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCLC at approximately 73 kDa. The expected band size for GCLC is at 73 kDa.
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