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ProductsBack/GCN2 Antibody - N-terminal region (Phospho-G11) (OAAB16897)

GCN2 Antibody - N-terminal region (Phospho-G11) (OAAB16897)

OAAB16897
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetEIF2AK4
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    GCN2 Antibody - N-terminal region (Phospho-G11) (OAAB16897)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Gene ID440275
  • Target name
    EIF2AK4
  • Target description
    eukaryotic translation initiation factor 2 alpha kinase 4
  • Target synonyms
    eIF-2-alpha kinase GCN2; GCN2; GCN2 eIF2alpha kinase; GCN2-like protein; general control nonderepressible 2; PVOD2
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EIF2AK4 using anti-EIF2AK4 antibody (A01172-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2AK4 antigen affinity purified polyclonal antibody (Catalog # A01172-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2AK4 at approximately 180-200KD. The expected band size for EIF2AK4 is at 187KD.
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