GCSF Receptor antibody [N2C1], Internal detects GCSF Receptor protein at cell membrane by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GCSF Receptor stained by GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109503/GTX109503_43712_20190927_WB_TPM_watermark_w_23060500_332.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109503/GTX109503_40506_20190809_WB_B_w_23060500_167.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![GCSF Receptor antibody [N2C1], Internal detects GCSF Receptor protein at secreted on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX109503/GTX109503_40506_20141128_IHC_w_23060500_600.webp "GCSF Receptor antibody [N2C1], Internal detects GCSF Receptor protein at secreted on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
GCSF Receptor antibody [N2C1], Internal detects GCSF Receptor protein at cell membrane by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GCSF Receptor stained by GCSF Receptor antibody [N2C1], Internal (GTX109503) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GCSF Receptor antibody [N2C1], Internal
GTX109503
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCSF3R
Overview
- SupplierGeneTex
- Product NameGCSF Receptor antibody [N2C1], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.36 mg/ml
- ConjugateUnconjugated
- Gene ID1441
- Target nameCSF3R
- Target descriptioncolony stimulating factor 3 receptor
- Target synonymsCD114, GCSFR, SCN7, granulocyte colony-stimulating factor receptor, CD114 antigen, G-CSF receptor, G-CSF-R, colony stimulating factor 3 receptor (granulocyte)
- HostRabbit
- IsotypeIgG
- Protein IDQ99062
- Protein NameGranulocyte colony-stimulating factor receptor
- Scientific DescriptionThe protein encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variants encoding four different isoforms have been found for this gene, with three of the isoforms being membrane-bound and the other being secreted and soluble. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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