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Western blot All lanes: GDAantibody at 1.24ug/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 52, 43, 53 kDa Observed band size: 36 kDa
Western blot All lanes: GDAantibody at 1.24ug/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 52, 43, 53 kDa Observed band size: 36 kDa
Western blot All lanes: GDAantibody at 1.24ug/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 52, 43, 53 kDa Observed band size: 36 kDa

GDA Antibody

CSB-PA009337ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGDA
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Overview

  • Supplier
    Cusabio
  • Product Name
    GDA Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9615
  • Target name
    GDA
  • Target description
    guanine deaminase
  • Target synonyms
    CYPIN; cytoplasmic PSD95 interactor; cytoplasmic PSD-95 interactor; GAH; GUANASE; guanine aminase; guanine aminohydrolase; guanine deaminase; NEDASIN; p51-nedasin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2T3
  • Protein Name
    Guanine deaminase
  • Scientific Description
    Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GDA using anti-GDA antibody (A01619-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat small intestine tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse small intestine tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GDA antigen affinity purified polyclonal antibody (Catalog # A01619-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GDA at approximately 51KD. The expected band size for GDA is at 51KD.
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