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IHC-P analysis of human brain tissue using GTX11115 GDNF Receptor alpha 1 antibody. Red : Primary antibody Blue : DAPI Dilution : 20 microg/ml
IHC-P analysis of human brain tissue using GTX11115 GDNF Receptor alpha 1 antibody. Red : Primary antibody Blue : DAPI Dilution : 20 microg/ml
IHC-P analysis of human brain tissue using GTX11115 GDNF Receptor alpha 1 antibody. Red : Primary antibody Blue : DAPI Dilution : 20 microg/ml

GDNF Receptor alpha 1 antibody

GTX11115
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetGFRA1
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Overview

  • Supplier
    GeneTex
  • Product Name
    GDNF Receptor alpha 1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 microg/mL. IHC-P: 1 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2674
  • Target name
    GFRA1
  • Target description
    GDNF family receptor alpha 1
  • Target synonyms
    GDNF family receptor alpha-1; GDNF receptor alpha 1d; GDNF receptor alpha 1e; GDNFR; GDNFRA; GDNFR-alpha-1; GFR-ALPHA-1; GFRalpha-1; Glial cell line-derived neurotrophic factor receptor alpha; GPI-linked anchor protein; PI-linked cell-surface accessory protein; RET ligand 1; RET1L; RETL1; TGF-beta-related neurotrophic factor receptor 1; TRNR1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP56159
  • Protein Name
    GDNF family receptor alpha-1
  • Scientific Description
    Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Feb 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GFRA1 using anti-GFRA1 antibody (PB9202). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human GFRA1 protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFRA1 antigen affinity purified polyclonal antibody (Catalog # PB9202) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFRA1 at approximately 39 kDa. The expected band size for GFRA1 is at 39 kDa.
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