More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/GFAP Antibody

GFAP Antibody

LS-C123602
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetGFAP
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GFAP Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Immunohistochemistry: 1:2000-1:5000 using 2% paraformaldehyde tissues. Immunocytochemistry: 1:2000-1:5000 using 2% paraformaldehyde cells. Western Blot: 1:10000-1:20000.. ICC (1:2000 - 1:5000), IHC (1:2000 - 1:5000), WB (1:10000 - 1:20000) Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Immunohistochemistry: 1:2000-1:5000 using 2% paraformaldehyde tissues. Immunocytochemistry: 1:2000-1:5000 using 2% paraformaldehyde cells. Western Blot: 1:10000-1:20000.
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2670
  • Target name
    GFAP
  • Target description
    glial fibrillary acidic protein
  • Target synonyms
    ALXDRD; glial fibrillary acidic protein
  • Host
    Chicken
  • Isotype
    IgY
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-GFAP Polyclonal Antibody (bs-0199R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Product group Antibodies
References
Bioss Antibodies
GFAP Polyclonal AntibodyBS-0199R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetGFAP
  • SizePrice
Product group Antibodies
Abbexa
Glial Fibrillary Acidic Protein (GFAP) AntibodyABX109813
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • SizePrice
Anti-GFAP Antibody
Product group Antibodies
Antibodies.com
Anti-GFAP AntibodyA270545
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
GFAP Antibody (Preservative Free)LS-C149200
ApplicationsELISA
TargetGFAP
  • SizePrice
Product group Antibodies
Biosynth
GFAP antibody10R-2253
  • SizePrice
Product group Antibodies
RayBiotech
Anti-GFAP Antibody130-10037
ApplicationsELISA
TargetGFAP
  • SizePrice
Western blot analysis of Rat Brain Tissue, diluted at 1:5000.
Product group Antibodies
Cusabio
GFAP Monoclonal AntibodyCSB-MA000216
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetGFAP
  • SizePrice
Product group Antibodies
Nordic-MUbio
Rabbit anti Glial fibrillary acidic protein (K39)MUB0700S
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetGFAP
  • SizePrice
Figure 1. Western blot analysis of GFAP using anti-GFAP antibody (PB9082). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C5 whole cell lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFAP antigen affinity purified polyclonal antibody (Catalog # PB9082) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFAP at approximately 50 kDa. The expected band size for GFAP is at 50 kDa.
Product group Antibodies
Boster Bio
Anti-GFAP Antibody Picoband(r)PB9082-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetGFAP
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-GFAPEB07478
ApplicationsWestern Blot, ELISA
TargetGFAP
  • SizePrice