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ProductsBack/Rabbit anti Glial fibrillary acidic protein (K39)

Rabbit anti Glial fibrillary acidic protein (K39)

MUB0700S
Nordic-MUbio
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGFAP
0.1 ml
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Glial fibrillary acidic protein (K39)
  • Delivery Days Customer
    7
  • Application Supplier Note
    K39 is suitable for immunoblotting, immunocytochemistry on permeabilized cells and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting;Immunocytochemistry
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID2670
  • Target name
    GFAP
  • Target description
    glial fibrillary acidic protein
  • Target synonyms
    ALXDRD; glial fibrillary acidic protein
  • Host
    Rabbit
  • Protein IDP14136
  • Protein Name
    Glial fibrillary acidic protein
  • Source
    K39 is a Rabbit polyclonal antiSerum derived by immunization of a Rabbit with a glial fibrillary acidic protein preparation from Human spinal cord.
  • Reactivity Supplier
    Human;Mouse;Rat;Zebrafish
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GFAP using anti-GFAP antibody (PB9082). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C5 whole cell lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFAP antigen affinity purified polyclonal antibody (Catalog # PB9082) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFAP at approximately 50 kDa. The expected band size for GFAP is at 50 kDa.
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