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Lane 1: Mouse Lung lysates; Lane 2: Mouse Lymph node lysates probed with GFI1/ZNF163 Polyclonal Antibody, Unconjugated (bs-13337R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lung lysates; Lane 2: Mouse Lymph node lysates probed with GFI1/ZNF163 Polyclonal Antibody, Unconjugated (bs-13337R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lung lysates; Lane 2: Mouse Lymph node lysates probed with GFI1/ZNF163 Polyclonal Antibody, Unconjugated (bs-13337R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

GFI1 Polyclonal Antibody

BS-13337R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetGFI1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    GFI1/ZNF163 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2672
  • Target name
    GFI1
  • Target description
    growth factor independent 1 transcriptional repressor
  • Target synonyms
    GFI-1; GFI1A; growth factor independence-1; growth factor independent 1 transcription repressor; growth factor independent protein 1; SCN2; zinc finger protein 163; zinc finger protein Gfi-1; ZNF163
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. IHC analysis of GFI1 using anti-GFI1 antibody (A00888-1). GFI1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-GFI1 Antibody (A00888-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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