Figure: Western blot of GFP recombinant protein detected with GeneTex monoclonal anti-GFP antibody. GFP recombinant protein was expressed in HeLa cells, where 50 ng (lane 1), 100 ng (lane 2) and 500 ng (lane 3) of lysate were loaded per lane. GTX21218 detects a 27 kDa band corresponding to the epitope tag GFP. The membrane was probed with the primary antibody diluted to 1.0 microg/ml for 1 h at room temperature followed by washes and reaction with a 1:2,500 dilution of IRDyeR 800 conjugated goat anti-Mouse IgG [H&L]. IRDyeR 800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
![IP analysis of recombinant GFP protein using GTX21218 GFP antibody [9F9.F9]. Lane 1 : Protein ladder Lane 2 : GFP input Antibody amount : 10 microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX21218/GTX21218_20240423_IP_20_24042320_789.webp "IP analysis of recombinant GFP protein using GTX21218 GFP antibody [9F9.F9]. Lane 1 : Protein ladder Lane 2 : GFP input Antibody amount : 10 microg")
![WB analysis of recombinant GFP protein using GTX21218 GFP antibody [9F9.F9]. Dilution : 1:400](https://www.genetex.com/upload/website/prouct_img/normal/GTX21218/GTX21218_20240423_WB_21_24042320_252.webp "WB analysis of recombinant GFP protein using GTX21218 GFP antibody [9F9.F9]. Dilution : 1:400")
![WB analysis of various samples using GTX21218 GFP antibody [9F9.F9]. Lane 1 : Protein ladder Lane 2 : 50 ng GFP Lane 3 : 20 ng GFP Lane 4 : 10 ng GFP Lane 5 : Mock Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX21218/GTX21218_20240423_WB_22_24042320_884.webp "WB analysis of various samples using GTX21218 GFP antibody [9F9.F9]. Lane 1 : Protein ladder Lane 2 : 50 ng GFP Lane 3 : 20 ng GFP Lane 4 : 10 ng GFP Lane 5 : Mock Dilution : 1:1000")
![WB analysis of recombinant GFP protein using GTX21218 GFP antibody [9F9.F9]. Lane 1 : Protein ladder Lane 2 : 50 ng GFP protein Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX21218/GTX21218_20240423_WB_23_24042320_715.webp "WB analysis of recombinant GFP protein using GTX21218 GFP antibody [9F9.F9]. Lane 1 : Protein ladder Lane 2 : 50 ng GFP protein Dilution : 1:1000")
Figure: Western blot of GFP recombinant protein detected with GeneTex monoclonal anti-GFP antibody. GFP recombinant protein was expressed in HeLa cells, where 50 ng (lane 1), 100 ng (lane 2) and 500 ng (lane 3) of lysate were loaded per lane. GTX21218 detects a 27 kDa band corresponding to the epitope tag GFP. The membrane was probed with the primary antibody diluted to 1.0 microg/ml for 1 h at room temperature followed by washes and reaction with a 1:2,500 dilution of IRDyeR 800 conjugated goat anti-Mouse IgG [H&L]. IRDyeR 800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
GFP antibody [9F9.F9]
GTX21218
ApplicationsDot Blot, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Other Application
Product group Antibodies
ReactivityJellyfish, Other Species
Overview
- SupplierGeneTex
- Product NameGFP antibody [9F9.F9]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:3000-1:30000. ICC/IF: Assay dependent. IHC-Fr: 1:1000-1:5000. FACS: Assay dependent. IP: Assay dependent. ELISA: 1:10000-1:30000. IHC: 1:1000-5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsDot Blot, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID9F9.F9
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG
- Scientific DescriptionThe jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expression and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
- ReactivityJellyfish, Other Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
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