Western Blot of Alkaline Phosphatase conjugated Goat anti-GFP antibody (GTX26661). Lane 1: GFP. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Block for 30 min RT. Predicted/Observed size: 28 kDa for GFP. Other band(s): none
Western Blot of Alkaline Phosphatase conjugated Goat anti-GFP antibody (GTX26661). Lane 1: GFP. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Block for 30 min RT. Predicted/Observed size: 28 kDa for GFP. Other band(s): none
GFP antibody (AP)
GTX26661
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityJellyfish, Other Species
Overview
- SupplierGeneTex
- Product NameGFP antibody (AP)
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2500. ICC/IF: 1:200-1:1000. ELISA: 1:2000-1:8000. IHC: 1:200-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateAlk. Phosphatase
- HostGoat
- IsotypeIgG
- Scientific DescriptionDesigned to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.
- ReactivityJellyfish, Other Species
- Storage Instruction2°C to 8°C
- UNSPSC12352203
References
- Pavoni E, Vaccaro P, D'Alessio V, et al. Simultaneous display of two large proteins on the head and tail of bacteriophage lambda. BMC Biotechnol. 2013,13:79. doi: 10.1186/1472-6750-13-79Read this paper