
The data was published in the journal EMBO J in 2019. PMID: 31368584
GFP antibody
GTX20290
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
Overview
- SupplierGeneTex
- Product NameGFP antibody
- Delivery Days Customer9
- ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- Scientific DescriptionA highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin (Mesaeli et al. 1999). In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Laspata N, Kaur P, Mersaoui SY, et al. PARP1 associates with R-loops to promote their resolution and genome stability. Nucleic Acids Res. 2023,51(5):2215-2237. doi: 10.1093/nar/gkad066Read this paper
- Simonetti B, Daly JL, Simón-Gracia L, et al. ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1. Proc Natl Acad Sci U S A. 2022,119(25):e2201980119. doi: 10.1073/pnas.2201980119Read this paper
- Simonetti B, Guo Q, Giménez-Andrés M, et al. SNX27-Retromer directly binds ESCPE-1 to transfer cargo proteins during endosomal recycling. PLoS Biol. 2022,20(4):e3001601. doi: 10.1371/journal.pbio.3001601Read this paper
- van Berkel AA, Santos TC, Shaweis H, et al. Loss of MUNC18-1 leads to retrograde transport defects in neurons. J Neurochem. 2021,157(3):450-466. doi: 10.1111/jnc.15256Read this paper
- Magic Z, Sandström J, Perez-Tenorio G. Ephrin‑B2 inhibits cell proliferation and motility in vitro and predicts longer metastasis‑free survival in breast cancer. Int J Oncol. 2019,55(6):1275-1286. doi: 10.3892/ijo.2019.4892Read this paper
- Brouwer M, Farzana F, Koopmans F, et al. SALM1 controls synapse development by promoting F-actin/PIP2-dependent Neurexin clustering. EMBO J. 2019,38(17):e101289. doi: 10.15252/embj.2018101289Read this paper
- Fouquerel E, Barnes RP, Uttam S, et al. Targeted and Persistent 8-Oxoguanine Base Damage at Telomeres Promotes Telomere Loss and Crisis. Mol Cell. 2019,75(1):117-130.e6. doi: 10.1016/j.molcel.2019.04.024Read this paper
- Borga C, Park G, Foster C, et al. Simultaneous B and T cell acute lymphoblastic leukemias in zebrafish driven by transgenic MYC: implications for oncogenesis and lymphopoiesis. Leukemia. 2019,33(2):333-347. doi: 10.1038/s41375-018-0226-6Read this paper
- Dominguez N, van Weering JRT, Borges R, et al. Dense-core vesicle biogenesis and exocytosis in neurons lacking chromogranins A and B. J Neurochem. 2018,144(3):241-254. doi: 10.1111/jnc.14263Read this paper
- Scanlon DP, Bah A, Krzeminski M, et al. An evolutionary switch in ND2 enables Src kinase regulation of NMDA receptors. Nat Commun. 2017,8:15220. doi: 10.1038/ncomms15220Read this paper




