GFP antibody
GTX20290
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityJellyfish, Other Species
Overview
- SupplierGeneTex
- Product NameGFP antibody
- Delivery Days Customer9
- Antibody SpecificityReactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP.
- ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- Scientific DescriptionA highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin (Mesaeli et al. 1999). In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos.
- ReactivityJellyfish, Other Species
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
- ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1. Simonetti B et al., 2022 Jun 21, Proc Natl Acad Sci U S ARead more
- SNX27-Retromer directly binds ESCPE-1 to transfer cargo proteins during endosomal recycling. Simonetti B et al., 2022 Apr, PLoS BiolRead more
- Loss of MUNC18-1 leads to retrograde transport defects in neurons. van Berkel AA et al., 2021 May, J NeurochemRead more
- Ephrin-B2 inhibits cell proliferation and motility in vitro and predicts longer metastasis-free survival in breast cancer. Magic Z et al., 2019 Dec, Int J OncolRead more
- SALM1 controls synapse development by promoting F-actin/PIP2-dependent Neurexin clustering. Brouwer M et al., 2019 Sep 2, EMBO JRead more
- Targeted and Persistent 8-Oxoguanine Base Damage at Telomeres Promotes Telomere Loss and Crisis. Fouquerel E et al., 2019 Jul 11, Mol CellRead more
- Simultaneous B and T cell acute lymphoblastic leukemias in zebrafish driven by transgenic MYC: implications for oncogenesis and lymphopoiesis. Borga C et al., 2019 Feb, LeukemiaRead more
- Dense-core vesicle biogenesis and exocytosis in neurons lacking chromogranins A and B. Dominguez N et al., 2018 Feb, J NeurochemRead more
- An evolutionary switch in ND2 enables Src kinase regulation of NMDA receptors. Scanlon DP et al., 2017 May 16, Nat CommunRead more
- Association of the breast cancer antiestrogen resistance protein 1 (BCAR1) and BCAR3 scaffolding proteins in cell signaling and antiestrogen resistance. Wallez Y et al., 2014 Apr 11, J Biol ChemRead more