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The data was published in the journal EMBO J in 2019. PMID: 31368584
The data was published in the journal EMBO J in 2019. PMID: 31368584
The data was published in the journal EMBO J in 2019. PMID: 31368584

GFP antibody

GTX20290
GeneTex
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
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Overview

  • Supplier
    GeneTex
  • Product Name
    GFP antibody
  • Delivery Days Customer
    9
  • Applications
    Gel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    A highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin (Mesaeli et al. 1999). In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Laspata N, Kaur P, Mersaoui SY, et al. PARP1 associates with R-loops to promote their resolution and genome stability. Nucleic Acids Res. 2023,51(5):2215-2237. doi: 10.1093/nar/gkad066
    Read this paper
  • Simonetti B, Daly JL, Simón-Gracia L, et al. ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1. Proc Natl Acad Sci U S A. 2022,119(25):e2201980119. doi: 10.1073/pnas.2201980119
    Read this paper
  • Simonetti B, Guo Q, Giménez-Andrés M, et al. SNX27-Retromer directly binds ESCPE-1 to transfer cargo proteins during endosomal recycling. PLoS Biol. 2022,20(4):e3001601. doi: 10.1371/journal.pbio.3001601
    Read this paper
  • van Berkel AA, Santos TC, Shaweis H, et al. Loss of MUNC18-1 leads to retrograde transport defects in neurons. J Neurochem. 2021,157(3):450-466. doi: 10.1111/jnc.15256
    Read this paper
  • Magic Z, Sandström J, Perez-Tenorio G. Ephrin‑B2 inhibits cell proliferation and motility in vitro and predicts longer metastasis‑free survival in breast cancer. Int J Oncol. 2019,55(6):1275-1286. doi: 10.3892/ijo.2019.4892
    Read this paper
  • Brouwer M, Farzana F, Koopmans F, et al. SALM1 controls synapse development by promoting F-actin/PIP2-dependent Neurexin clustering. EMBO J. 2019,38(17):e101289. doi: 10.15252/embj.2018101289
    Read this paper
  • Fouquerel E, Barnes RP, Uttam S, et al. Targeted and Persistent 8-Oxoguanine Base Damage at Telomeres Promotes Telomere Loss and Crisis. Mol Cell. 2019,75(1):117-130.e6. doi: 10.1016/j.molcel.2019.04.024
    Read this paper
  • Borga C, Park G, Foster C, et al. Simultaneous B and T cell acute lymphoblastic leukemias in zebrafish driven by transgenic MYC: implications for oncogenesis and lymphopoiesis. Leukemia. 2019,33(2):333-347. doi: 10.1038/s41375-018-0226-6
    Read this paper
  • Dominguez N, van Weering JRT, Borges R, et al. Dense-core vesicle biogenesis and exocytosis in neurons lacking chromogranins A and B. J Neurochem. 2018,144(3):241-254. doi: 10.1111/jnc.14263
    Read this paper
  • Scanlon DP, Bah A, Krzeminski M, et al. An evolutionary switch in ND2 enables Src kinase regulation of NMDA receptors. Nat Commun. 2017,8:15220. doi: 10.1038/ncomms15220
    Read this paper