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WB analysis of various sample lysates using GTX64638 GFPT1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64638 GFPT1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64638 GFPT1 antibody. Dilution : 1:1000 Loading : 25microg per lane

GFPT1 antibody

GTX64638
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGFPT1
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Overview

  • Supplier
    GeneTex
  • Product Name
    GFPT1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:200 - 1:2000. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2673
  • Target name
    GFPT1
  • Target description
    glutamine--fructose-6-phosphate transaminase 1
  • Target synonyms
    CMS12, CMSTA1, GFA, GFAT, GFAT 1, GFAT1, GFAT1m, GFPT, GFPT1L, MSLG, glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1, D-fructose-6-phosphate amidotransferase 1, glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1, glutamine:fructose-6-phosphate amidotransferase 1, hexosephosphate aminotransferase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ06210
  • Protein Name
    Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1
  • Scientific Description
    This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. [provided by RefSeq, Sep 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of GFPT1 using anti-GFPT1 antibody (A04341-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFPT1 antigen affinity purified polyclonal antibody (Catalog # A04341-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFPT1 at approximately 79 kDa. The expected band size for GFPT1 is at 79 kDa.
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