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ProductsBack/GILT (G-11): m-IgGkappa BP-HRP Bundle

GILT (G-11): m-IgGkappa BP-HRP Bundle

SC-523969
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetIFI30
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    GILT (G-11): m-IgGkappa BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10437
  • Target name
    IFI30
  • Target description
    IFI30 lysosomal thiol reductase
  • Target synonyms
    GILT, IFI-30, IP-30, IP30, gamma-interferon-inducible lysosomal thiol reductase, gamma-interferon-inducible protein IP-30, interferon gamma-inducible protein 30 preproprotein, interferon, gamma-inducible protein 30, legumaturain
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IFI30 using anti-IFI30 antibody (A05754-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFI30 antigen affinity purified polyclonal antibody (Catalog # A05754-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFI30 at approximately 23 kDa. The expected band size for IFI30 is at 28 kDa.
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