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Western Blot analysis of RAW264.7 cells using GIMAP4 Polyclonal Antibody
Western Blot analysis of RAW264.7 cells using GIMAP4 Polyclonal Antibody
Western Blot analysis of RAW264.7 cells using GIMAP4 Polyclonal Antibody

GIMAP4 Antibody

CSB-PA002681
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGIMAP4
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Overview

  • Supplier
    Cusabio
  • Product Name
    GIMAP4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55303
  • Target name
    GIMAP4
  • Target description
    GTPase, IMAP family member 4
  • Target synonyms
    IAN-1, IAN1, IMAP4, MSTP062, GTPase IMAP family member 4, human immune associated nucleotide 1, immune-associated nucleotide-binding protein 1, immunity associated protein 4, immunity-associated nucleotide 1 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NUV9
  • Protein Name
    GTPase IMAP family member 4
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GIMAP4 using anti-GIMAP4 antibody (A10738-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIMAP4 antigen affinity purified polyclonal antibody (Catalog # A10738-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIMAP4 at approximately 38 kDa. The expected band size for GIMAP4 is at 38 kDa.
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