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GIMAP4 Antibody (OAAB18376)

OAAB18376
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetGIMAP4
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    GIMAP4 Antibody (OAAB18376)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Format
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Gene ID55303
  • Target name
    GIMAP4
  • Target description
    GTPase, IMAP family member 4
  • Target synonyms
    GTPase IMAP family member 4; human immune associated nucleotide 1; IAN1; IAN-1; IMAP4; immune-associated nucleotide-binding protein 1; immunity associated protein 4; immunity-associated nucleotide 1 protein; MSTP062
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GIMAP4 using anti-GIMAP4 antibody (A10738-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIMAP4 antigen affinity purified polyclonal antibody (Catalog # A10738-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIMAP4 at approximately 38 kDa. The expected band size for GIMAP4 is at 38 kDa.
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