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GINS3 Polyclonal Antibody

RD219175A
Reddot Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetGINS3
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    GINS3 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.6 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64785
  • Target name
    GINS3
  • Target description
    GINS complex subunit 3
  • Target synonyms
    PSF3, DNA replication complex GINS protein PSF3, GINS complex subunit 3 (Psf3 homolog)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BRX5
  • Protein Name
    DNA replication complex GINS protein PSF3
  • Scientific Description
    This gene encodes a protein subunit of the GINS heterotetrameric complex, which is essential for the initiation of DNA replication and replisome progression in eukaryotes. Alternatively spliced transcript variants encoding distinct isoforms have been described. - This is a GINS3 Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GINS3 using anti-GINS3 antibody (A10458-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GINS3 antigen affinity purified polyclonal antibody (Catalog # A10458-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GINS3 at approximately 25 kDa. The expected band size for GINS3 is at 25 kDa.
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