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ProductsBack/GJA4 / CX37 / Connexin 37 Antibody

GJA4 / CX37 / Connexin 37 Antibody

LS-C14545
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGJA4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GJA4 / CX37 / Connexin 37 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Suitable for use in ELISA, Western Blot. Western Blot: We recommend testing of antiserum at 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure using Chemiluminescence technique. ELISA (1:10K-100K using 50-100 ng control peptide/well.. ELISA, WB (1:1000 - 1:5000) Suitable for use in ELISA, Western Blot. Western Blot: We recommend testing of antiserum at 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure using Chemiluminescence technique. ELISA (1:10K-100K using 50-100 ng control peptide/well.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2701
  • Target name
    GJA4
  • Target description
    gap junction protein alpha 4
  • Target synonyms
    connexin-37; CX37; gap junction alpha-4 protein; gap junction protein, alpha 4, 37kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GJA4 using anti-GJA4 antibody (A05569-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJA4 antigen affinity purified polyclonal antibody (Catalog # A05569-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJA4 at approximately 37 kDa. The expected band size for GJA4 is at 37 kDa.
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