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Western Blot analysis of A549 cells using Connexin 47 Polyclonal Antibody
Western Blot analysis of A549 cells using Connexin 47 Polyclonal Antibody
Western Blot analysis of A549 cells using Connexin 47 Polyclonal Antibody

GJC2 Antibody

CSB-PA040043
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetGJC2
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Overview

  • Supplier
    Cusabio
  • Product Name
    GJC2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57165
  • Target name
    GJC2
  • Target description
    gap junction protein gamma 2
  • Target synonyms
    CX46.6, Cx47, GJA12, HLD2, LMPH1C, LMPHM3, PMLDAR, SPG44, gap junction gamma-2 protein, connexin-46.6, connexin-47, gap junction alpha-12 protein, gap junction protein, gamma 2, 47kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5T442
  • Protein Name
    Gap junction gamma-2 protein
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GJC2 using anti-GJC2 antibody (PB9706). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: U20S Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJC2 antigen affinity purified polyclonal antibody (Catalog # PB9706) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJC2 at approximately 47 kDa. The expected band size for GJC2 is at 47 kDa.
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