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Western Blot analysis of Jurkat cells using GK1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using GK1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using GK1 Polyclonal Antibody

GK Antibody

CSB-PA002694
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGK
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Overview

  • Supplier
    Cusabio
  • Product Name
    GK Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2710
  • Target name
    GK
  • Target description
    glycerol kinase
  • Target synonyms
    ATP:glycerol 3-phosphotransferase; GK1; GKD; glycerokinase; glycerol kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32189
  • Protein Name
    Glycerol kinase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Glycerol kinase using anti-Glycerol kinase antibody (A02884-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SW579 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: rat testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glycerol kinase antigen affinity purified polyclonal antibody (Catalog # A02884-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glycerol kinase at approximately 61KD. The expected band size for Glycerol kinase is at 61KD.
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