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GK / Glycerol Kinase Antibody

LS-C185074
LifeSpan BioSciences
ApplicationsImmunoDiffusion, ImmunoElectrophoresis, Western Blot, ELISA
Product group Antibodies
TargetGK
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GK / Glycerol Kinase Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoDiffusion, ImmunoElectrophoresis, Western Blot, ELISA
  • Applications Supplier
    ELISA, ID, IE, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2710
  • Target name
    GK
  • Target description
    glycerol kinase
  • Target synonyms
    ATP:glycerol 3-phosphotransferase; GK1; GKD; glycerokinase; glycerol kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Glycerol kinase using anti-Glycerol kinase antibody (A02884-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SW579 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: rat testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glycerol kinase antigen affinity purified polyclonal antibody (Catalog # A02884-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glycerol kinase at approximately 61KD. The expected band size for Glycerol kinase is at 61KD.
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