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Western blot All lanes: GK5 antibody at 4microg/ml + K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60, 34, 28 kDa Observed band size: 60 kDa
Western blot All lanes: GK5 antibody at 4microg/ml + K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60, 34, 28 kDa Observed band size: 60 kDa
Western blot All lanes: GK5 antibody at 4microg/ml + K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60, 34, 28 kDa Observed band size: 60 kDa

GK5 Antibody

CSB-PA744414LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetGK5
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Overview

  • Supplier
    Cusabio
  • Product Name
    GK5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID256356
  • Target name
    GK5
  • Target description
    glycerol kinase 5
  • Target synonyms
    glycerol kinase 5, ATP:glycerol 3-phosphotransferase 5, GK 5, glycerokinase 5, glycerol kinase 5 (putative), putative glycerol kinase 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6ZS86
  • Protein Name
    Glycerol kinase 5
  • Scientific Description
    cytoplasm, mitochondrion, glycerol kinase activity, glycerol metabolic process, glycerol-3-phosphate biosynthetic process, phosphorylation, triglyceride metabolic process
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GK5 using anti-GK5 antibody (A15997). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GK5 antigen affinity purified polyclonal antibody (Catalog # A15997) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GK5 at approximately 59 kDa. The expected band size for GK5 is at 59 kDa.
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