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ProductsBack/GKAP1 Antibody (clone 3G4)

GKAP1 Antibody (clone 3G4)

LS-C169388
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGKAP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GKAP1 Antibody (clone 3G4)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA (1:10000), IF (1:200 - 1:1000), IHC, IHC-P (1:200 - 1:1000), WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3G4
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID80318
  • Target name
    GKAP1
  • Target description
    G kinase anchoring protein 1
  • Target synonyms
    cGMP-dependent protein kinase anchoring protein 42kDa; cGMP-dependent protein kinase-anchoring protein of 42 kDa; FKSG21; G kinase-anchoring protein 1; GKAP42; protein kinase anchoring protein GKAP42
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GKAP1 using anti-GKAP1 antibody (A14397-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GKAP1 antigen affinity purified polyclonal antibody (Catalog # A14397-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GKAP1 at approximately 40-42 kDa. The expected band size for GKAP1 is at 42 kDa.
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