Gli1 antibody [HL27] detects Gli1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Gli1 stained by Gli1 antibody [HL27] (GTX635619) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Gli1 antibody [HL247] detects Gli1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Gli1 stained by Gli1 antibody [HL247] (GTX635619) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635619/GTX635619_44020_20200814_IHC-P_M_w_23061202_168.webp "Gli1 antibody [HL247] detects Gli1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Gli1 stained by Gli1 antibody [HL247] (GTX635619) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Non-transfected (–) and transfected (+) RMS13 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635619/GTX635619_44020_20201008_WB_shRNA_watermark_w_23061202_411.webp "Non-transfected (–) and transfected (+) RMS13 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635619/GTX635619_44020_20210924_WB_B_w_23061202_513.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Gli1 antibody [HL247] detects Gli1 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Gli1 stained by Gli1 antibody [HL247] (GTX635619) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX635619/GTX635619_44550_20230721_ICC_IF_B_23072519_127.webp "Gli1 antibody [HL247] detects Gli1 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Gli1 stained by Gli1 antibody [HL247] (GTX635619) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![RMS 13 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635619/GTX635619_44648_20231013_WB_Fraction_23101921_286.webp "RMS 13 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Immunoprecipitation of Gli1 protein from RMS 13 whole cell extract using 5 μg of Gli1 antibody [HL247] (GTX635619). Western blot analysis was performed using Gli1 antibody [HL247] (GTX635619). EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).](https://www.genetex.com/upload/website/prouct_img/normal/GTX635619/GTX635619_45257_20240419_IP_24042400_416.webp "Immunoprecipitation of Gli1 protein from RMS 13 whole cell extract using 5 μg of Gli1 antibody [HL247] (GTX635619). Western blot analysis was performed using Gli1 antibody [HL247] (GTX635619). EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).")
![Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL27] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635619/GTX635619_45257_20231215_WB_24080602_247.webp "Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL27] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Gli1 antibody [HL27] detects Gli1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Gli1 stained by Gli1 antibody [HL27] (GTX635619) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Gli1 antibody [HL247]
GTX635619
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGli1
Overview
- SupplierGeneTex
- Product NameGli1 antibody [HL247]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL247
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID14632
- Target nameGli1
- Target descriptionGLI-Kruppel family member GLI1
- Target synonymsZfp-5, Zfp5, zinc finger protein GLI1, glioma-associated oncogene homolog, zinc finger protein 5
- HostRabbit
- IsotypeIgG
- Protein IDP47806
- Protein NameZinc finger protein GLI1
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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- SizePrice