Western Blot of Rabbit anti-Gli-3 antibody. Lane 1: 50 kDa molecular weight marker. Lane 2: 293T cells transfected with CrkL-Flag. Lane 3: 293T cells transfected with human Gli-3. Load: 35 μg per lane. Primary antibody: Gli-3 antibody at 1:400 for overnight at 4oC. Secondary antibody: IRDye800? rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4oC. Predicted/Observed size: 170-190 kDa for hGli-3. Other band(s): Non specific background ~60kDa.

GLI3 antibody

GTX26050

ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application

Product group Antibodies

ReactivityFish, Human

TargetGLI3

Overview

Supplier
GeneTex
Product Name
GLI3 antibody
Delivery Days Customer
9
Application Supplier Note
WB: 1:500-1:2000. IHC-P: 0.5-5 microg/mL. ELISA: 1:6000-1:30000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Applications
ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Certification
Research Use Only
Clonality
Polyclonal
Concentration
1 mg/ml
Conjugate
Unconjugated
Gene ID2737
Target name
GLI3
Target description
GLI family zinc finger 3
Target synonyms
ACLS, GCPS, GLI3-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV, transcriptional activator GLI3, GLI-Kruppel family member GLI3, glioma-associated oncogene family zinc finger 3, oncogene GLI3, zinc finger protein GLI3
Host
Rabbit
Isotype
IgG
Protein IDP10071
Protein Name
Transcriptional activator GLI3
Scientific Description
Gli3 is belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli3 is also expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS); an autosomal dominant disorder affecting limb and craniofacial development.
Reactivity
Fish, Human
Storage Instruction
-20°C or -80°C,2°C to 8°C
UNSPSC
12352203

References

Li N, Truong S, Nouri M, et al. Non-canonical activation of hedgehog in prostate cancer cells mediated by the interaction of transcriptionally active androgen receptor proteins with Gli3. Oncogene. 2018,37(17):2313-2325. doi: 10.1038/s41388-017-0098-7
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Hunt R, Bragina O, Drews M, et al. Generation and characterization of mouse monoclonal antibody 5E1 against human transcription factor GLI3. Hybridoma (Larchmt). 2007,26(4):231-40.
Read this paper
Hunt R, Bragina O, Drews M, et al. Generation and characterization of mouse monoclonal antibody 5E1 against human transcription factor GLI3. Hybridoma (Larchmt). 2007,26(3):131-9.
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GLI3 antibody

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Overview

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