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ProductsBack/GLOD4 antibody [N1C3]

GLOD4 antibody [N1C3]

GTX104484
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGLOD4
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Overview

  • Supplier
    GeneTex
  • Product Name
    GLOD4 antibody [N1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51031
  • Target name
    GLOD4
  • Target description
    glyoxalase domain containing 4
  • Target synonyms
    C17orf25, CGI-150, HC6, HC71, glyoxalase domain-containing protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HC38
  • Protein Name
    Glyoxalase domain-containing protein 4
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Kim J, Kang J, Kang YL, et al. Ketohexokinase-A acts as a nuclear protein kinase that mediates fructose-induced metastasis in breast cancer. Nat Commun. 2020,11(1):5436. doi: 10.1038/s41467-020-19263-1
    Read this paper

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Figure 1. Western blot analysis of GLOD4 using anti-GLOD4 antibody (A13315-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse stomach tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLOD4 antigen affinity purified polyclonal antibody (A13315-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLOD4 at approximately 35 kDa. The expected band size for GLOD4 is at 35 kDa.
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