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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA009521EA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA009521EA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA009521EA01HU at dilution of 1:100

GLRX Antibody

CSB-PA009521EA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGLRX
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Overview

  • Supplier
    Cusabio
  • Product Name
    GLRX Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2745
  • Target name
    GLRX
  • Target description
    glutaredoxin
  • Target synonyms
    glutaredoxin-1; GRX; GRX1; thioltransferase; thioltransferase-1; TTase-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35754
  • Protein Name
    Glutaredoxin-1
  • Scientific Description
    Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Glutaredoxin 1/GLRX using anti-Glutaredoxin 1/GLRX antibody (A03392-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glutaredoxin 1/GLRX antigen affinity purified polyclonal antibody (Catalog # A03392-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glutaredoxin 1/GLRX at approximately 12 kDa. The expected band size for Glutaredoxin 1/GLRX is at 12 kDa.
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