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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA878876DSR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA878876DSR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA878876DSR1HU at dilution of 1:100

GLRX2 Antibody

CSB-PA878876DSR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGLRX2
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Overview

  • Supplier
    Cusabio
  • Product Name
    GLRX2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51022
  • Target name
    GLRX2
  • Target description
    glutaredoxin 2
  • Target synonyms
    CGI-133, GRX2, glutaredoxin 2, bA101E13.1 (GRX2 glutaredoxin (thioltransferase) 2), glutaredoxin (thioltransferase) 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NS18
  • Protein Name
    Glutaredoxin-2, mitochondrial
  • Scientific Description
    Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRX2/GLRX2 using anti-GRX2/GLRX2 antibody (A05331). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRX2/GLRX2 antigen affinity purified polyclonal antibody (Catalog # A05331) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRX2/GLRX2 at approximately 18KD. The expected band size for GRX2/GLRX2 is at 18KD.
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