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GLS2 / Glutaminase 2 Antibody

LS-C144338
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGLS2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GLS2 / Glutaminase 2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. Western Blot: Predicted: 36, 55, 62, 66 kDa Observed: 55, 62 kDa. ELISA, IF, IHC, WB (0.5 - 1 µg/ml) GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. Western Blot: Predicted: 36, 55, 62, 66 kDa Observed: 55, 62 kDa IHC-P
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID27165
  • Target name
    GLS2
  • Target description
    glutaminase 2
  • Target synonyms
    breast cell glutaminase; GA; GLS; glutaminase 2 (liver, mitochondrial); glutaminase I; glutaminase liver isoform, mitochondrial; hLGA; LGA; L-glutamine amidohydrolase; phosphate-activated glutaminase; phosphate-dependent glutaminase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GLS2 using anti-GLS2 antibody (A05334-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLS2 antigen affinity purified polyclonal antibody (Catalog # A05334-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLS2 at approximately 66 kDa. The expected band size for GLS2 is at 66 kDa.
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