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Formalin-fixed and paraffin embedded rat brain labeled with Anti-GLS2/LGA Polyclonal Antibody, Unconjugated (bs-13376R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-GLS2/LGA Polyclonal Antibody, Unconjugated (bs-13376R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-GLS2/LGA Polyclonal Antibody, Unconjugated (bs-13376R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

GLS2 Polyclonal Antibody

BS-13376R
Bioss Antibodies
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetGLS2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    GLS2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID27165
  • Target name
    GLS2
  • Target description
    glutaminase 2
  • Target synonyms
    breast cell glutaminase; GA; GLS; glutaminase 2 (liver, mitochondrial); glutaminase I; glutaminase liver isoform, mitochondrial; hLGA; LGA; L-glutamine amidohydrolase; phosphate-activated glutaminase; phosphate-dependent glutaminase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UI32
  • Protein Name
    Glutaminase liver isoform, mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GLS2 using anti-GLS2 antibody (A05334-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLS2 antigen affinity purified polyclonal antibody (Catalog # A05334-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLS2 at approximately 66 kDa. The expected band size for GLS2 is at 66 kDa.
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