Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with GLT25D2 Polyclonal Antibody, Unconjugated (bs-13379R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.