FACS analysis of Hela cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
![FACS analysis of Jurkat cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22768/GTX22768_133_FACS_w_23060620_224.webp "FACS analysis of Jurkat cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature")
![FACS analysis of NIH/3T3 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue). Dilution : 2 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22768/GTX22768_134_FACS_w_23060620_524.webp "FACS analysis of NIH/3T3 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue). Dilution : 2 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature")
![WB analysis of mouse liver extract using GTX22768 Glucocorticoid Receptor antibody [BuGR2].](https://www.genetex.com/upload/website/prouct_img/normal/GTX22768/GTX22768_1586_WB_w_23060620_941.webp "WB analysis of mouse liver extract using GTX22768 Glucocorticoid Receptor antibody [BuGR2].")
![ICC/IF analysis of A549 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22768/GTX22768_410_ICC-IF_w_23060620_543.webp "ICC/IF analysis of A549 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of HeLa cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22768/GTX22768_411_ICC-IF_w_23060620_802.webp "ICC/IF analysis of HeLa cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of U251 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22768/GTX22768_412_ICC-IF_w_23060620_451.webp "ICC/IF analysis of U251 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
FACS analysis of Hela cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
Glucocorticoid Receptor antibody [BuGR2]
GTX22768
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityGuinea Pig, Human, Mouse, Primate, Rabbit, Rat, Sheep, Xenopus, Yeast
TargetNr3c1
Overview
- SupplierGeneTex
- Product NameGlucocorticoid Receptor antibody [BuGR2]
- Delivery Days Customer9
- Antibody SpecificityUsing enzymatic digestion analysis, this antibody reacts with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.
- Application Supplier NoteWB: 5 microg/ml. ICC/IF: 1:50-1:500. IHC-P: 5 microg/ml. FACS: 1-2 microg/test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDBuGR2
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID24413
- Target nameNr3c1
- Target descriptionnuclear receptor subfamily 3, group C, member 1
- Target synonymsGcr; glucocorticoid receptor; GR; Grl
- HostMouse
- IsotypeIgG2a
- Protein IDP06536
- Protein NameGlucocorticoid receptor
- Scientific Descriptionglucocorticoid receptor that binds and activates hormone-dependent transcriptional enhancers [RGD, Feb 2006]
- ReactivityGuinea Pig, Human, Mouse, Primate, Rabbit, Rat, Sheep, Xenopus, Yeast
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203