FACS analysis of Hela cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
![FACS analysis of Jurkat cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22768/GTX22768_133_FACS_w_23060620_224.webp "FACS analysis of Jurkat cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature")
![FACS analysis of NIH/3T3 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue). Dilution : 2 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22768/GTX22768_134_FACS_w_23060620_524.webp "FACS analysis of NIH/3T3 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue). Dilution : 2 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature")
![WB analysis of mouse liver extract using GTX22768 Glucocorticoid Receptor antibody [BuGR2].](https://www.genetex.com/upload/website/prouct_img/normal/GTX22768/GTX22768_1586_WB_w_23060620_941.webp "WB analysis of mouse liver extract using GTX22768 Glucocorticoid Receptor antibody [BuGR2].")
![ICC/IF analysis of A549 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22768/GTX22768_410_ICC-IF_w_23060620_543.webp "ICC/IF analysis of A549 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of HeLa cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22768/GTX22768_411_ICC-IF_w_23060620_802.webp "ICC/IF analysis of HeLa cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of U251 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22768/GTX22768_412_ICC-IF_w_23060620_451.webp "ICC/IF analysis of U251 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
FACS analysis of Hela cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
Glucocorticoid Receptor antibody [BuGR2]
GTX22768
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNr3c1
Overview
- SupplierGeneTex
- Product NameGlucocorticoid Receptor antibody [BuGR2]
- Delivery Days Customer9
- Application Supplier NoteWB: 5 microg/ml. ICC/IF: 1:50-1:500. IHC-P: 5 microg/ml. FACS: 1-2 microg/test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDBuGR2
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID24413
- Target nameNr3c1
- Target descriptionnuclear receptor subfamily 3, group C, member 1
- Target synonymsGR, Gcr, Grl, glucocorticoid receptor
- HostMouse
- IsotypeIgG2a
- Protein IDP06536
- Protein NameGlucocorticoid receptor
- Scientific Descriptionglucocorticoid receptor that binds and activates hormone-dependent transcriptional enhancers [RGD, Feb 2006]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
Related products
Product group Antibodies
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetNr3c1
- SizePrice