Glucose dehydrogenase (GDH) Assay Kit (Colorimetric)

LifeSpan BioSciences

Glucose dehydrogenase (GDH) Assay Kit (Colorimetric)

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For quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism.

Spectrophotometry (visible) For quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism.

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GLUCOSE DEHYDROGENASE (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway. This non-radioactive, colorimetric GDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

-20°C

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