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Immunohistochemistry analysis of paraffin-embedded human brain using GLUT-1 Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded human brain using GLUT-1 Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded human brain using GLUT-1 Polyclonal Antibody at dilution of 1:300.

GLUT-1 Polyclonal Antibody

E-AB-70090
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    GLUT-1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    GLUT1,also known as SLC2A1,is an ubiquitously expressed glucose transporter and responsible for the basal level of glucose uptake in most cell types. Human erythrocytes express the highest level of the GLUT1. Defects in SLC2A1 are the cause of GLUT1 deficiency syndrome type 1 and type 2. High expression of GLUT1 has been reported to be a reliable immunohistochemical marker for juvenile hemangiomas. GLUT1 protein may appear as two or more distinct forms among 43 kDa to 55 kDa due to the different glycosylation state. And the conversion of highly glycosylated form of GLUT1 to less glycosylated form has been reported to correlate to differentiation. (8263524,23302780)
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate, Lane 3: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A1 antigen affinity purified polyclonal antibody (Catalog # PB9435) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A1 at approximately 55KD. The expected band size for SLC2A1 is at 55KD.
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