Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
GLUT12 Polyclonal Antibody
BS-2540R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSLC2A12
Overview
- SupplierBioss
- Product NameGLUT12 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID154091
- Target nameSLC2A12
- Target descriptionsolute carrier family 2 member 12
- Target synonymsGLUT12, GLUT8, solute carrier family 2, facilitated glucose transporter member 12, GLUT-12, glucose transporter type 12, solute carrier family 2 (facilitated glucose transporter), member 12
- HostRabbit
- IsotypeIgG
- Protein IDQ8TD20
- Protein NameSolute carrier family 2, facilitated glucose transporter member 12
- Storage Instruction-20°C
- UNSPSC12352203
References
- Gil-Iturbe E, Solas M, Cuadrado-Tejedo M, et al. GLUT12 Expression in Brain of Mouse Models of Alzheimer's Disease. Mol Neurobiol. 2020,57(2):798-805. doi: 10.1007/s12035-019-01743-1Read this paper
- Gil-Iturbe E, Castilla-Madrigal R, Barrenetxe J, et al. GLUT12 expression and regulation in murine small intestine and human Caco-2 cells. J Cell Physiol. 2019,234(4):4396-4408. doi: 10.1002/jcp.27231Read this paper
- Murakami R, Chiba Y, Tsuboi K, et al. Immunoreactivity of glucose transporter 8 is localized in the epithelial cells of the choroid plexus and in ependymal cells. Histochem Cell Biol. 2016,146(2):231-6. doi: 10.1007/s00418-016-1444-5Read this paper
Datasheet
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