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K562 cells probed with GLUT4 Polyclonal Antibody, unconjugated (bs-0384R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
K562 cells probed with GLUT4 Polyclonal Antibody, unconjugated (bs-0384R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
K562 cells probed with GLUT4 Polyclonal Antibody, unconjugated (bs-0384R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

GLUT4 Polyclonal Antibody

BS-0384R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSLC2A4
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Overview

  • Supplier
    Bioss
  • Product Name
    GLUT4 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6517
  • Target name
    SLC2A4
  • Target description
    solute carrier family 2 member 4
  • Target synonyms
    GLUT4, solute carrier family 2, facilitated glucose transporter member 4, GLUT-4, glucose transporter type 4, insulin-responsive, insulin-responsive glucose transporter type 4, solute carrier family 2 (facilitated glucose transporter), member 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14672
  • Protein Name
    Solute carrier family 2, facilitated glucose transporter member 4
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Ye X, Shen Y, Ni C, et al. Irisin reverses insulin resistance in C2C12 cells via the p38-MAPK-PGC-1α pathway. Peptides. 2019,119:170120. doi: 10.1016/j.peptides.2019.170120
    Read this paper
  • Zhang T, Deng B, Zhang R, et al. Dietary Sea Buckthorn Pomace Induces Beige Adipocyte Formation in Inguinal White Adipose Tissue in Lambs. Animals (Basel). 2019,9(4). doi: 10.3390/ani9040193
    Read this paper
  • Sikder K, Shukla SK, Patel N, et al. High Fat Diet Upregulates Fatty Acid Oxidation and Ketogenesis via Intervention of PPAR-γ. Cell Physiol Biochem. 2018,48(3):1317-1331. doi: 10.1159/000492091
    Read this paper
  • Zhang S, Yang Q, Ren M, et al. Effects of isoleucine on glucose uptake through the enhancement of muscular membrane concentrations of GLUT1 and GLUT4 and intestinal membrane concentrations of Na+/glucose co-transporter 1 (SGLT-1) and GLUT2. Br J Nutr. 2016,116(4):593-602. doi: 10.1017/S0007114516002439
    Read this paper

Datasheet

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Figure 1. Western blot analysis of GLUT4 using anti-GLUT4 antibody (PB9109). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat cardiac muscle tissue lysate, lane 2: rat skeletal muscle tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUT4 antigen affinity purified polyclonal antibody (Catalog # PB9109) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUT4 at approximately 55KD. The expected band size for GLUT4 is at 55KD.
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