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ProductsBack/GLYAT Antibody - middle region (OAAB09357)

GLYAT Antibody - middle region (OAAB09357)

OAAB09357
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Human
TargetGLYAT
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    GLYAT Antibody - middle region (OAAB09357)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Gene ID10249
  • Target name
    GLYAT
  • Target description
    glycine-N-acyltransferase
  • Target synonyms
    ACGNAT, CAT, GAT, glycine N-acyltransferase, AAc, HRP-1(CLP), acyl-CoA:glycine N-acyltransferase, aralkyl acyl-CoA N-acyltransferase, aralkyl acyl-CoA:amino acid N-acyltransferase, aralkyl-CoA N-acyltransferase, benzoyl-coenzyme A:glycine N-acyltransferase, epididymis secretory sperm binding protein, glycine N-benzoyltransferase
  • Host
    Rabbit
  • Reactivity
    Bovine, Human
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GLYAT using anti-GLYAT antibody (A09506-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLYAT antigen affinity purified polyclonal antibody (Catalog # A09506-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLYAT at approximately 34 kDa. The expected band size for GLYAT is at 34 kDa.
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