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Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Polyclonal Antibody

CAU25045
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetGapdh
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Overview

  • Supplier
    Biomatik
  • Product Name
    Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID14433
  • Target name
    Gapdh
  • Target description
    glyceraldehyde-3-phosphate dehydrogenase
  • Target synonyms
    Gapd, glyceraldehyde-3-phosphate dehydrogenase, peptidyl-cysteine S-nitrosylase GAPDH
  • Host
    Rabbit
  • Protein IDP16858
  • Protein Name
    Glyceraldehyde-3-phosphate dehydrogenase
  • Scientific Description
    The Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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This image was generously provided by Loyda M. Meléndez at the University of Puerto Rico Medical Sciences Campus.\nA total of 30ug of monocyte-derived macrophage lysates were reduced and denatured in 1X Laemli Buffer with beta-mercaptoethanol. Samples were loaded into a TGX 4-20% gel, at 150V for 1 hour. Proteins were transferred to a PVDF membrane and probed for proteins of interest. The membrane was re-probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) in 5% milk at 1:250 dilution overnight at 4°C. The next day, the membrane was washed with TBS-T and incubated with goat anti-rabbit HRP conjugated secondary antibody for 1 hour at room temperature. The membrane was exposed for 18 seconds.\n
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