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GMPS / GMP Synthase Antibody

LS-C334825
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGMPS
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GMPS / GMP Synthase Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8833
  • Target name
    GMPS
  • Target description
    guanine monophosphate synthase
  • Target synonyms
    GATD7, GMP synthase [glutamine-hydrolyzing], GMP synthase, GMP synthetase, MLL/GMPS fusion protein, glutamine amidotransferase, guanine monophosphate synthetase, guanosine 5'-monophosphate synthase, testicular tissue protein Li 82
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: GMPS antibody at 2.69microg/ml Lane 1: Hela whole cell lysate Lane 2: HL60 whole cell lysate Lane 3: MCF-7 whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/10000 dilution Predicted band size: 77, 66 kDa Observed band size: 77 kDa
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Figure 1. Western blot analysis of GMPS using anti-GMPS antibody (A03643-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human COLO320 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human Jurkat whole cell lysates, Lane 8: human K562 whole cell lysates, Lane 9: rat PC-12 whole cell lysates, Lane 10: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GMPS antigen affinity purified polyclonal antibody (Catalog # A03643-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GMPS at approximately 77KD. The expected band size for GMPS is at 77KD.
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