Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638001/GTX638001_T-44900_20230120_WB_M_R_23013122_506.webp "Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638001/GTX638001_44977_20230317_WB_23032022_623.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![GNAI1 + GNAI2 + GNAI3 antibody [HL2093] detects GNAI1 + GNAI2 + GNAI3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GNAI1 + GNAI2 + GNAI3 stained by GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638001/GTX638001_T-44900_20230414_ICC_IF_23041719_188.webp "GNAI1 + GNAI2 + GNAI3 antibody [HL2093] detects GNAI1 + GNAI2 + GNAI3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GNAI1 + GNAI2 + GNAI3 stained by GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.")
![Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638001/GTX638001_44977_20230609_WB_Z_23061400_840.webp "Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole Japanese medaka extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638001/GTX638001_44977_20250815_WB_medaka_25082121_769.webp "Whole Japanese medaka extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GNAI1 + GNAI2 + GNAI3 antibody [HL2093]
GTX638001
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetGNAI1
Overview
- SupplierGeneTex
- Product NameGNAI1 + GNAI2 + GNAI3 antibody [HL2093]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2093
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2770
- Target nameGNAI1
- Target descriptionG protein subunit alpha i1
- Target synonymsGi, HG1B, NEDHISB, guanine nucleotide-binding protein G(i) subunit alpha-1, Gi1 protein alpha subunit, adenylate cyclase-inhibiting G alpha protein, guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1, heterotrimeric guanine nucleotide-binding protein 1B
- HostRabbit
- IsotypeIgG
- Protein IDP04899
- Protein NameGuanine nucleotide-binding protein G(i) subunit alpha-2
- ReactivityHuman, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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