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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA009611LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA009611LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA009611LA01HU at dilution of 1:100

GNG11 Antibody

CSB-PA009611LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGNG11
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Overview

  • Supplier
    Cusabio
  • Product Name
    GNG11 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2791
  • Target name
    GNG11
  • Target description
    G protein subunit gamma 11
  • Target synonyms
    GNGT11, HG3H1, guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-11, G protein gamma-11 subunit, guanine nucleotide binding protein (G protein), gamma 11, guanine nucleotide-binding protein G(I)/G(S)/G(O) gamma-11 subunit, heterotrimeric guanine nucleotide-binding protein 3H1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61952
  • Protein Name
    Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-11
  • Scientific Description
    Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GNG11 using anti-GNG11 antibody (A13707). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNG11 antigen affinity purified polyclonal antibody (Catalog # A13707) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNG11 at approximately 10KD. The expected band size for GNG11 is at 10KD.
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