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Gng4 Polyclonal Antibody

CAC10366
Biomatik
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGNG4
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Overview

  • Supplier
    Biomatik
  • Product Name
    GNG4 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2786
  • Target name
    GNG4
  • Target description
    G protein subunit gamma 4
  • Target synonyms
    guanine nucleotide binding protein (G protein), gamma 4; guanine nucleotide binding protein 4; guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50150
  • Protein Name
    Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4
  • Scientific Description
    The Gng4 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, IHC, IF.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GNG4 using anti-GNG4 antibody (A13925-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNG4 antigen affinity purified polyclonal antibody (Catalog # A13925-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNG4 at approximately 12KD. The expected band size for GNG4 is at 12KD.
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