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Goat anti-ABCA4

EB08615
Everest Biotech
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
TargetABCA4
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ABCA4 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the Endoplasmic Reticulum of MCF7 and HeLa cells. Recommended concentration: 10microg/ml.
  • Applications
    ImmunoFluorescence, ELISA
  • Applications Supplier
    Pep-ELISA, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID24
  • Target name
    ABCA4
  • Target description
    ATP binding cassette subfamily A member 4
  • Target synonyms
    ABC10; ABCR; ARMD2; ATP binding cassette transporter; ATP-binding cassette sub-family A member 4; ATP-binding cassette transporter, retinal-specific; ATP-binding cassette, sub-family A (ABC1), member 4; ATP-binding transporter, retina-specific; CORD3; FFM; photoreceptor rim protein; retinal-specific ATP-binding cassette transporter; retinal-specific phospholipid-transporting ATPase ABCA4; retina-specific ABC transporter; RIM ABC transporter; RIM protein; RMP; RP19; stargardt disease protein; STGD; STGD1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000341.2. Purification: Antigen affinity purified. Names and symbols: ABCA4; ATP-binding cassette, sub-family A (ABC1), member 4; ABC10; ABCR; ARMD2; CORD3; DKFZp781N1972; FFM; RMP; RP19; STGD; STGD1; ATP binding cassette transporter; ATP-binding cassette, sub-family A member 4; ATP-binding transporter, retina-specific; ret
  • Reactivity Supplier
    Human, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ABCA4 using anti-ABCA4 antibody (A01048). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat eye tissue lysates, Lane 3: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCA4 antigen affinity purified polyclonal antibody (Catalog # A01048) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCA4 at approximately 256-300 kDa. The expected band size for ABCA4 is at 256 kDa.
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