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Goat anti-ACPP / PAP

EB09390
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetACP3
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ACPP / PAP Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence:: This antibody has been successfully used in IF on Mouse: Nousiainen HO et al. (2014) PMID: 24846136.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID55
  • Target name
    ACP3
  • Target description
    acid phosphatase 3
  • Target synonyms
    5'-NT; acid phosphatase, prostate; ACP-3; ACPP; ecto-5'-nucleotidase; prostatic acid phosphatase; prostatic acid phosphotase; protein tyrosine phosphatase ACP3; thiamine monophosphatase; TM-PAP; TMPase
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001090.2. GeneIDs all Nonhuman: 56318 (mouse); 56780 (rat);. Purification: Antigen affinity purified. Names and symbols: ACPP; acid phosphatase, prostate; ACP-3; ACP3; PAP; prostatic acid phosphotase
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Mice deficient in transmembrane prostatic acid phosphatase display increased GABAergic transmission and neurological alterations. Nousiainen HO et al., 2014, PLoS One
    Read more

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Figure 1. Western blot analysis of PAP/ACP3 using anti-PAP/ACP3 antibody (A02082-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAP/ACP3 antigen affinity purified polyclonal antibody (Catalog # A02082-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAP/ACP3 at approximately 45-50 kDa. The expected band size for PAP/ACP3 is at 45 kDa.
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