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ProductsBack/Goat anti-Amphiphysin / AMPH

Goat anti-Amphiphysin / AMPH

EB07245
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetAMPH
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Amphiphysin / AMPH Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID273
  • Target name
    AMPH
  • Target description
    amphiphysin
  • Target synonyms
    AMPH1, amphiphysin, amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen), amphiphysin I
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001626.1; NP_647477.1. GeneIDs all Nonhuman: 218038 (mouse);. Purification: Antigen affinity purified. Names and symbols: AMPH; amphiphysin; amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen) ; HGNC:471; AMPH1
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Amphiphysin/AMPH using anti-Amphiphysin/AMPH antibody (A02364-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiphysin/AMPH antigen affinity purified polyclonal antibody (Catalog # A02364-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiphysin/AMPH at approximately 115-125 kDa. The expected band size for Amphiphysin/AMPH is at 76 kDa.
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