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Goat anti-Androgen Receptor

EB06441
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetAR
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Androgen Receptor Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10microg/ml. Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using EB06441 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.
  • Applications
    ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, IF, ChIP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID367
  • Target name
    AR
  • Target description
    androgen receptor
  • Target synonyms
    AIS; androgen receptor; AR8; DHTR; dihydrotestosterone receptor; HUMARA; HYSP1; KD; NR3C4; nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; TFM
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000035.2. GeneIDs all Nonhuman: 11835 (mouse); 24208 (rat);. Purification: Antigen affinity purified. Names and symbols: AR; androgen receptor; dihydrotestosterone receptor; KD; AIS; TFM; DHTR; SBMA; NR3C4; SMAX1; HUMARA; HYSP1; androgen receptor; dihydrotestosterone receptor; RP11-383C12.1
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Androgen Receptor/AR using anti-Androgen Receptor/AR antibody (A00542). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human 22RV1 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Androgen Receptor/AR antigen affinity purified polyclonal antibody (Catalog # A00542) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Androgen Receptor/AR at approximately 120KD. The expected band size for Androgen Receptor/AR is at 120KD.
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