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Goat anti-ANGPT1

EB10272
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetANGPT1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ANGPT1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the plasma membranes of A431 and HeLa cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, WB-Trf, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID284
  • Target name
    ANGPT1
  • Target description
    angiopoietin 1
  • Target synonyms
    AGP1; AGPT; ANG1; ANG-1; angiopoietin-1; HAE5
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001137.2. GeneIDs all Nonhuman: 11600 (mouse); 89807 (rat);. Purification: Antigen affinity purified. Names and symbols: ANGPT1; angiopoietin 1; AGP1; AGPT; ANG1
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Angiopoietin-1 using anti-Angiopoietin-1 antibody (A00853). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human ANGPT1 protein 1ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin-1 antigen affinity purified polyclonal antibody (Catalog # A00853) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiopoietin-1 at approximately 70KD. The expected band size for Angiopoietin-1 is at 57KD.
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